金朴消积颗粒通过调控HIF-1α/VEGF/iNOS信号轴及重塑肠道菌群改善功能性便秘小鼠症状的机制研究

Mechanism of Jinpu Xiaoji Granules in Ameliorating Functional Constipation in Mice via Regulation of the HIF-1α/VEGF/iNOS Signaling Axis and Remodeling of the Gut Microbiota

    摘要
  • 摘要:
    目的 评价金朴消积颗粒对洛哌丁胺诱导小鼠功能性便秘模型（Functional constipation，FC）的改善作用，并探索其潜在机制。
    方法 建立小鼠FC模型，结合金朴消积颗粒化学成分以及网络药理学分析潜在通路，观察给药后胃肠功能变化，检测结肠组织中HIF-1α、VEGF、iNOS表达，并分析肠道菌群组成。
    结果 JPXJ含有多种活性成分，可能通过HIF-1信号通路发挥作用。JPXJ中剂量能有效改善便秘小鼠的体质量、粪便性状和肠道推进功能，下调结肠HIF-1α、VEGF、iNOS表达，并调节乳酸杆菌等有益菌群的丰度。
    结论 本研究初步证实金朴消积颗粒对儿童功能性便秘具有治疗潜力，其作用可能与调节HIF-1信号通路及改善肠道微生态有关，为拓展其临床应用于儿童FC提供了实验依据。

     

    Abstract:
    OBJECTIVE To evaluate the ameliorative effects of Jinpu Xiaoji (JPXJ) granules on loperamide-induced functional constipation (FC) in mice and to explore the underlying mechanisms.
    METHODS A mouse model of FC was established, and the chemical components of JPXJ were analyzed alongside network pharmacology to identify potential pathways. Gastrointestinal function was observed after administration, and the expression of HIF-1α, VEGF, and iNOS in colon tissue was measured. Changes in gut microbiota composition were also analyzed.
    RESULTS JPXJ contains multiple active components and may exert effects via the HIF-1 signaling pathway. The medium dose of JPXJ effectively improved body weight, fecal characteristics, and intestinal propulsion in constipated mice, downregulated the expression of HIF-1α, VEGF, and iNOS in colon tissue, and modulated the abundance of beneficial bacteria such as Lactobacillus.
    CONCLUSION This study preliminarily demonstrates the potential of JPXJ granules in treating FC, possibly through regulating the HIF-1 signaling pathway and improving gut microbiota, providing experimental support for its expanded use in pediatric FC.

     

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